| Title | Micropropagation of guava (Psidium guajava) seedlings, a plant with interest in cool subtropics, using an innovative BB culture medium |
| Publication Type | Journal Papers |
| Year of Publication | 2023 |
| Authors | Thiresia-Teresa Tzatzani, Ioanna Michail, Anastasia Boutsika, Eirini Sarrou & Ioannis Ganopoulos |
| Journal | Biotechnology & Biotechnological Equipment |
| Volume | VOL. 37, NO.1 |
| Pagination | 139–150 |
| ISSN | |
| URL | |
| DOI | 10.1080/13102818.2022.2159524 |
| Citation Key | |
| Keywords | Psidium guajava; BB basal medium; clonal propagation; micropropagation protocol; genetic diversity; polymorphism |
| Abstract | Psidium guajava is a new crop cultivated in Greece, with a potential pharmaceutical and
ornamental uses. An efficient and reliable protocol for in vitro clonal propagation of P. guajava
from seeds was used. Five different media were examined. Shoot proliferation succeeded on a
new basal medium (BB) supplemented with a modified vitamin solution (vit BB), without plant
growth regulators. In addition, shoots on media supplemented with BB vitamin solution performed
better than in Murashige and Scoog (MS) and Woody Plant (WP) media. When BB medium was
used, the greatest shoot number resulted in 4 mg L−1 6-benzyladenine (BA) with a score of 2.8.
Regenerated single shoots were rooted in the BB medium, supplemented with 0.1 ΝΑΑ (mg
L−1) and producing 2.2 roots of 4.6 cm, along with 3.1 cm of adventitious shoots. Acclimatization
was accomplished in three phases, using peat-moss, vermiculite and perlite (1:1:1), with a total
survival of 83.3%. To examine the genetic diversity of the plant material, Inter Simple Sequence
Repeat (ISSR) and Start Codon Targeted (SCoT) molecular markers were used. The results indicated
a low diversity, along with high percentage of polymorphism for both ISSR and SCoT analyzes. |
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